Cell culture models for peptide research

Standard immortalized and primary cell lines used in in-vitro peptide studies.

Common cell lines in peptide research: - **C2C12** — mouse myoblasts (IGF-1, growth-axis studies) - **HUVEC** — human umbilical vein endothelial cells (angiogenesis) - **HepG2** — human hepatoma (metabolic, mitochondrial studies) - **3T3 / NIH-3T3** — mouse fibroblasts (wound healing, migration) - **Primary dermal fibroblasts** — human skin cells (collagen, GHK-Cu) - **CHO-K1 (recombinant)** — Chinese hamster ovary cells stably expressing human receptors (GLP-1R, GIPR, GCGR) Each cell line has specific media requirements, passage limits, and receptor expression profiles that must be characterized before peptide treatment.

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