Mass spectrometry (ESI-MS) for peptide identity

Electrospray ionization mass spectrometry confirms peptide molecular weight within ±1 Da.

ESI-MS ionizes peptides in solution and measures their mass-to-charge ratio (m/z). For identity confirmation: - The observed monoisotopic mass must match the theoretical mass within ±1 Da - Multiple charge states (+2, +3, +4) provide redundant confirmation - Tandem MS (MS/MS) can sequence the peptide by fragmenting it Janoshik uses ESI-MS for every Aurex batch. A mass-spec "match" means the observed mass falls within tolerance of the theoretical mass calculated from the amino-acid sequence. This is complementary to HPLC-UV: HPLC measures purity, MS confirms identity.

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