HPLC-UV purity analysis

High-performance liquid chromatography with UV detection — the gold standard for peptide purity quantification.

HPLC-UV separates peptide components by reversed-phase chromatography and detects them via UV absorbance at 214 nm (peptide bond wavelength). Key readouts: - Main-peak purity (% of total integrated area) - Minor peaks (degradation products, synthesis impurities) - Retention time (identity confirmation) Aurex's purity floor is ≥98.4% main peak, verified independently by Janoshik Analytical. HPLC-UV at 214 nm captures all peptide bonds but does not distinguish between peptides with similar sequences — that's where mass spectrometry complements the analysis.

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