Longevity · Research reference

NAD+ 1000mg (buffered)

High-dose buffered NAD+ — single vial. Nicotinamide adenine dinucleotide — buffered formulation, 1000mg per vial. Researched for mitochondrial NAD+/NADH ratio, sirtuin signaling, and cellular energetics pathways.

See the wiki entry

Half-life

varies — IV / SC protocols

Molar mass

663.43 g/mol

Purity floor

≥99%

CAS

53-84-9

Mechanism

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to redox biology and a substrate for sirtuins (SIRT1–7), PARPs, and CD38. The buffered form maintains stability at physiological pH for cell-culture supplementation.

Common in-vitro applications

Sirtuin activation studies
SIRT1/SIRT3 deacetylase activity assays + downstream substrate (FOXO, p53) acetylation Westerns.
Working range: 100 µM to 1 mM
PARP-1 activity modulation
DNA-damage models with PARP-1 cleavage / NAD+ depletion readouts.
Working range: 50 µM to 500 µM
Redox state measurement
NAD+/NADH ratio quantification in mitochondrial respiration assays.
Working range: 100 µM to 2 mM

Reconstitution + storage

Reconstitution

10ml bacteriostatic water per vial

Per-vial mass

1000mg

Lyophilized storage

−20°C or below, desiccated

Reconstituted storage

2–8°C, use within 14 days (NAD+ oxidizes in solution)

Light + air

Protect from light and air

Solubility

Soluble in bacteriostatic water; prepare fresh aliquots for each experiment

Background: lyophilization · reconstitution

Co-treatment partners

MOTS-c
Parallel mitochondrial-energetics readouts.
Resveratrol / NMN
Sirtuin-pathway agonist comparators.

Selected peer-reviewed citations

Houtkooper RH, et al. (2012). NAD+ in cellular metabolism and disease. Endocrine Reviews.
Imai S, Guarente L (2014). NAD+ and sirtuins in aging. Trends in Cell Biology.

Verification

Read the COA

Open the batch lookup — Janoshik HPLC-UV + ESI-MS packet.

Open

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NAD+ 1000mg (buffered) · $58

Lyophilized, COA-verified, tracked US shipping.

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Reference data is supplied for in-vitro literature context only. Aurex does not provide dosage or use guidance — Federal regulations governing research-chemical distribution prohibit it. NAD+ 1000mg (buffered) is sold for in-vitro research applications only, by qualified researchers. Not for human or veterinary use. Not FDA approved.