NAD+ 1000mg (buffered)

Mechanism

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to redox biology and a substrate for sirtuins (SIRT1–7), PARPs, and CD38. The buffered form maintains stability at physiological pH for cell-culture supplementation.

In-vitro applications

• Sirtuin activation studies
  SIRT1/SIRT3 deacetylase activity assays + downstream substrate (FOXO, p53) acetylation Westerns.
  Working range: 100 µM to 1 mM
• PARP-1 activity modulation
  DNA-damage models with PARP-1 cleavage / NAD+ depletion readouts.
  Working range: 50 µM to 500 µM
• Redox state measurement
  NAD+/NADH ratio quantification in mitochondrial respiration assays.
  Working range: 100 µM to 2 mM

Researchers commonly co-treat with

• MOTS-c — Parallel mitochondrial-energetics readouts.
• Resveratrol / NMN — Sirtuin-pathway agonist comparators.

Storage and handling

• · Lyophilized: −20°C or below, desiccated
• · Reconstituted: 2–8°C, use within 14 days (NAD+ oxidizes in solution)
• · Light + air: Protect from light and air
• · Solubility: Soluble in bacteriostatic water; prepare fresh aliquots for each experiment

See also: [[lyophilization]] · [[reconstitution]]

Selected peer-reviewed citations

1. Houtkooper RH, et al. (2012). NAD+ in cellular metabolism and disease. Endocrine Reviews.
2. Imai S, Guarente L (2014). NAD+ and sirtuins in aging. Trends in Cell Biology.