GHK-Cu 50mg

Mechanism

GHK-Cu is the copper(II) complex of the endogenous tripeptide glycyl-L-histidyl-L-lysine. In vitro it acts as a high-affinity copper carrier with characterized activity on fibroblast proliferation, decorin and collagen-I/III synthesis, MMP-2 activation, and antioxidant gene expression. Pickart and colleagues have shown the Cu(II) complex modulates ~4,000 human genes toward a regenerative profile in cultured fibroblasts.

In-vitro applications

• Dermal fibroblast ECM synthesis
  Collagen-I, collagen-III, decorin, and elastin upregulation assays in primary human dermal fibroblast cultures.
  Working range: 1 nM to 10 µM
• Wound-bed angiogenesis modeling
  VEGF / FGF-2 release + tube-formation assays in HUVEC + co-culture wound models.
  Working range: 10 nM to 1 µM
• Hair-follicle dermal papilla proliferation
  BrdU incorporation + hair-shaft elongation in cultured dermal papilla cells and follicle organ explants.
  Working range: 0.1 µM to 10 µM

Researchers commonly co-treat with

• BPC-157 — Parallel evaluation in fibroblast migration + angiogenesis pathway readouts — complementary mechanisms (NO/VEGF axis vs copper-carrier).
• Argireline (Acetyl Hexapeptide-3) — Pair surface-applied SNAP-25 modulator with dermal ECM-remodeling reference in skin-equivalent assays.
• Thymosin Beta-4 (TB-500 / Peptide-2 axis) — Co-treatment in scratch-wound + actin-binding assays; shared interest in dermal regeneration pathways.

Storage and handling

• · Lyophilized: −20°C or below, desiccated
• · Reconstituted: 2–8°C, use within 30 days
• · Light + air: Protect from light — copper complex is photosensitive in solution
• · Solubility: Soluble in bacteriostatic water; reconstituted solutions exhibit a characteristic deep blue color from the Cu(II) chromophore

See also: [[lyophilization]] · [[reconstitution]]

Selected peer-reviewed citations

1. Pickart L, Margolina A (2018). Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data. International Journal of Molecular Sciences.
2. Pickart L, Vasquez-Soltero JM, Margolina A (2015). GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration. Oxidative Medicine and Cellular Longevity.
3. Maquart FX, et al. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu²⁺. FEBS Letters.